Large Language Models (LLMs) have received significant attention due to their potential to transform the field of education and assessment through the provision of automated responses to a diverse range of inquiries. The objective of this research is to examine the efficacy of three LLMs - ChatGPT, BingChat, and Bard - in relation to their performance on the Vietnamese High School Biology Examination dataset. This dataset consists of a wide range of biology questions that vary in difficulty and context. By conducting a thorough analysis, we are able to reveal the merits and drawbacks of each LLM, thereby providing valuable insights for their successful incorporation into educational platforms. This study examines the proficiency of LLMs in various levels of questioning, namely Knowledge, Comprehension, Application, and High Application. The findings of the study reveal complex and subtle patterns in performance. The versatility of ChatGPT is evident as it showcases potential across multiple levels. Nevertheless, it encounters difficulties in maintaining consistency and effectively addressing complex application queries. BingChat and Bard demonstrate strong performance in tasks related to factual recall, comprehension, and interpretation, indicating their effectiveness in facilitating fundamental learning. Additional investigation encompasses educational environments. The analysis indicates that the utilization of BingChat and Bard has the potential to augment factual and comprehension learning experiences. However, it is crucial to acknowledge the indispensable significance of human expertise in tackling complex application inquiries. The research conducted emphasizes the importance of adopting a well-rounded approach to the integration of LLMs, taking into account their capabilities while also recognizing their limitations. The refinement of LLM capabilities and the resolution of challenges in addressing advanced application scenarios can be achieved through collaboration among educators, developers, and AI researchers.