This paper proposes hierarchical support vector machines for weapon Identification using images of repeated stab wound patterns caused by sharp metal weapons used in homicidal cases and also presents a comparative study with standard flat support vector machine. The methodology includes the segmentation technique for the extraction of region-of-interest in the image using transition region-based segmentation algorithm and then texture, shape and size features were extracted from the segmented image. For multiple classes, a hierarchical support vector machine is adapted as a classifier. This approach gives a computationally interesting and efficient alternative solution to identify the weapons used in the crime; this method uses the digital images of repeated stab wound patterns which appear on the human body. The experimental study has three main stages, at the first stage includes generating of non-overlapping segments from the pattern, at the second stage the features of wound patterns are extracted and finally identification of patterns and its weapon of cause. The proposed method accuracy assessment is performed and also comparison study is performed with standard flat support vector machine and with the traditional method of forensic pathology. The experimental results achieved for Identification is 96.71% accuracy, with an available database of 500 images of a pattern consisting of repeated stabbed wounds. From the comparative study, the proposed methodology has given better results than standard SVM and traditional method. The proposed method delivers a better solution for identification from the image of the repeated stab wound pattern as there is no human intervention that reduces the error and data manipulation unlike traditional manual method.