Construction of Eukaryotic recombinant expression Vector pCI-ORF5-ORF6/ pCI-ORF5-ORF6B-Hsp70 Containing Hsp70 and PRRSV GP5/M (encoded by ORF5 and ORF6 genes), and to study its immune effect. After being identified by enzyme analysis and nucleotide sequencing test, the repression vector plasmid was transfected into COS-7 cells. The transient expression protein was detected by Western-blotting. The immunogenicities of this DNA vaccine constructs were firstly investigated in a mouse moder. IFN-γ, IL-4 of cytokine, and the spleen T-lymphocyte subgroup quantity (CD4+/CD8+) were detected, DNA vaccine distribution in mice by PCR .The result shows that the recombinant plasmid pCI-ORF5-ORF6-Hsp70 could induce higher response of cellular immune responses and specific immune responses in mouse, the DNA Vaccines in mice Vaccinated via as heart and liver ,lung and kidney, muscle and brain each time step after immunity. providing the clinical basic data and theoretical basis for success of the DNA vaccine development.