Plant is a gift of almighty to the living being in the earth. Leaf is an essential component for any types of plant including crops, fruit and vegetables. Before the scheduled decay of the leaf due to deficiency there are patches of dead zone spot or sections generally visible. This paper introduces a novel image based analysis to identify patches of dead zone spot or sections generally visible due to deficiency. Clustering, colour object based segmentation and colour transformation techniques using significant salient features identification are applied over 12 plant leaves collected naturally from gardens and crop fields. Hue, saturation and Value based and L*a*b* colour model based object analysis is being applied over diseased leaf and portion of leaf to identify the dead zone automatically. Derivative based edge analysis is being applied to identify the outline edge and dead zone segmentation in leaf image. K-means clustering has played an important role to cluster dead zone using colour based object area segmentation.

Correlation between gene expression profiles to disease or different developmental stages of a cell through microarray data and its analysis has been a great deal in molecular biology. As the microarray data have thousands of genes and very few sample, thus efficient feature extraction and computational method development is necessary for the analysis. In this paper we have proposed an effective feature extraction method based on factor analysis (FA) with discrete wavelet transform (DWT) to detect informative genes. Radial basis function neural network (RBFNN) classifier is used to efficiently predict the sample class which has a low complexity than other classifier. The potential of the proposed approach is evaluated through an exhaustive study by many benchmark datasets. The experimental results show that the proposed method can be a useful approach for cancer classification.