Automatic Number Plate Recognition (ANPR) is becoming very popular and topic of research for the Intelligent Transportation System (ITS). Many researchers are working in this direction, as it is the topic of interest. In proposed system, we have presented a novel approach for number plate (NP) detection, which can be useful for Indian conditions. The system works well in different illumination conditions and 24 hours manner. Experiments achieved excellent accuracy of 98.88% of overall accuracy of NP detection on 90 vehicle images with different conditions and captured at different timing during day and night. Out of these 90 images, 89 images were segmented successfully. The minimum image size was 800 X 600 pixels. The system was developed using the Microsoft .NET 3.5 framework and Visual Studio 2008 as IDE with the Intel core i3 2.13 GHz processor having 3 GB RAM. Other systems discussed in this paper reported better processing time of less than 1s, but some of these systems work under restricted conditions and accuracy is also not as good as our system.