The objective for this research is to assess the optimal measurement model for cell adhesion force. The Human Umbilical Vein Endothelial Cell Line (ECV304) was cultured on a type of biomedical material, polydimethylsiloxane (PDMS). The research also studied the parameters such as alternatives of working solution, styles of PDMS substrate, driving frequency and collagen smearing etc. The result showed the cells cultured on the large area substrate with 2 mm structural spacing and the small area substrate with 100μm structural spacing have better adhesive force. It was also clear to find that large area substrates also showed faster cell growth and expansion. They are more suitable as culture substrates for the measurement of cell adhesion force. As for work solution, 2% glucose solution that has relative low conductivity and concentration has the best measurement that effectively obtained cell adhesion force.