Knowledge transfer is the foundation of group scientific and research activities, and the life line of the knowledge innovation of science and research group. However, there are various difficulties for the internal knowledge transfer in the science and research group of high technology enterprises, which hinders the improvement of the knowledge innovation and performance of the science and research groups of high technology enterprises. The knowledge transfer situation in the science and research groups of high technology enterprises is worrying. The key of the management of the science and research group in high technology enterprises is the cooperation among members. For the group operation, only mutual trust is not sufficient. There should be effective encouraging and restriction mechanism to motivate members’ enthusiasm of knowledge transfer and avoid members’ moral risks.