This paper proposes a design of fractal multi-band microstrip patch antenna dedicated for wireless applications. The proposed antenna uses a FR-4 epoxy substrate having permittivity 4.4 and tangent loss to be 0.02.The microstrip antenna proposed is designed by incorporating double I-shape slot on the surface of rectangular patch and simulated by using HFSS software. Further the antenna is modified by inserting fractal shape and obtained the three frequency bands located at 2.4Ghz , 3.5Ghz and 5.50Ghz respectively and antenna parameter like VSWR, return loss, antenna gain, directivity of an antenna and bandwidth has been analyzed. The designing of proposed antenna is to be followed by fabrication by using A FR-4 substrate of thickness 1.60mm. Further, this paper discusses, the simulated and measured results comparison and analysis of the same. For designing and simulating of antennae simulation software HFSS has been used. The simulated and measured results represented are in permissible range.