Enhancing network performance has been studied by a number of researchers. The need to provide greater throughput on network infrastructure has been the key driver for these studies. The use of jumbo frames is considered one of the methodologies that can be employed to increase data throughput on networks. In this research undertaking, the authors implement jumbo frames on a test-bed implemented with Windows Server 2003/2008 networks and performance related metrics are measured for both IPv4 and IPv6 implementations. The results obtained in this empirical study shows that performance metrics values are different in various scenarios.