Researches on the relation of a loaded mechanical energy to the damaged banana maturation and its quality evolution has significance to its storage, but there are few researches at present. This paper studied on the effect of the loaded energy on banana to its internal maturation substance evolution and stored quality with time, including the change of maximum impact stress, re-enduring impact energy. The results showed there existed a significant correlation between energy transformation and increment of maturity substance in the damaged banana. Also, there existed a significant association between energy transformation and increment of maturity substance in the damaged banana in its storage time, and appeared a trends that the mechanical energy being transferred into fruit internal energy increased its maturity substance and decreased its stored quality, but individual maturity difference had less effect. Thus, decreasing the energy and the substance transformation in damage area are the important means of extending the physiology life of fruits, which is an urgent problem to be solved in their field of processing and storage at present.