The Internet of Things (IoT) driven Industrial Revolution 4.0 (IR4.0) and this is impacting every sector of the global economy. With IoT devices, everything is computerized. Today's digital forensics is no longer limited to computers, mobiles, or networks. The current digital forensics landscape demands a significantly different approach. The traditional digital forensics frameworks no longer meet the current requirements. Therefore, in this paper, we propose a novel framework called “Multi-level Artifact of Interest Digital Forensics Framework for IoT” (MAoIDFF-IoT). The keynote "Multi-level" aims to cover all levels of the IoT architecture. Our novel IoT digital forensics framework focuses on the Artifact of Interest (AoI). Additionally, it proposes the action/detection matrix. It encompasses the advantages of the previous frameworks while introducing new features specifically designed to make the framework suitable for current and future IoT investigation scenarios. The MAoIDFF-IoT framework is designed to face the challenges of IoT forensic analysis and address the diverse architecture of IoT environments. Our proposed framework was evaluated through real scenario experiments. The evaluation of the experimental results reveals the superiority of our framework over existing frameworks in terms of usability, inclusivity, focus on the (AoI), and acceleration of the investigation process.